Clostridium difficile Testing

Clostridium difficile Testing

C. difficile Testing Background

  • As of September 2016 the microbiology lab will abandon routine Clostridium difficile PCR testing and adopt the C. diff Quik Chek Complete® EIA test
  • The C. diff Quik Chek Complete® EIA test utilizes antibodies to detect glutamate dehydrogenase (GDH) (also known as the antigen common to both toxigenic and non-toxigenic C. diff) and Toxins A/B

C. diff Quik Chek Complete® EIA Test

GDH or Ag will be positive if C. diff (toxigenic or non-toxigenic) is present

Toxin A/B will be positive when toxigenic C. diff is actively producing toxin

General Interpretation of Results:

  • Ag(+)/Toxin(+) = C. difficile Infection
  • Ag(-)/Toxin(-) = Absence of C. difficile Infection
  • Ag(+)/Toxin(-) = C. difficile carrier status
    • Ag(+)/Toxin(-) specimens will be held by microlab for 48hrs should a provider wish to call and request a PCR test
  • Sensitivity: 90.5%
  • Specificity: 93.1%
  • NPV: 97.6%
  • Sensitivity: 87.8%
  • Specificity: 99.4%
  • NPV: 98.1%