Confirm patient ID and print labels
Wash hands and don gloves
Apply tourniquet to locate vein
Clean with CHG swap (Prevantics)
Scrub for 1 minute, dry for 30 sec
Remove Caps from culture bottles and disinfect with alcohol swab
Use a 20 mL syringe with 21-gauge needle for adults
Using aseptic technique, attach needle to syringe
Perform venipuncture and collect 16-20mL
Safely dispose the needle and attach blood culture transfer device to syringe
IF air in syringe, let air get to top of syringe
Fill anaerobic (purple) bottle FIRST with 8-10 mL
Fill aerobic (blue) bottle SECOND with 8-10 mL
Invert bottles 4-5 times to mix thoroughly